Computational design of second-site suppressor mutations at protein-protein interfaces
Sammond, D. W., Eletr, Z. M., Purbeck, C. and Kuhlman, B. Proteins 78:1055-1065
Metal templated design of protein interfaces
Salgado, E. N., Ambroggio, X. I., Brodin, J. D., Lewis, R. A., Kuhlman, B. and Tezcan, F. A. Proc Natl Acad Sci U S A 107:1827-1832.
Rosetta.design3.1computational protein design software
Rosetta design can be used to identify sequences compatible with a given protein backbone. Some of Rosetta design's successes include the design of a novel protein fold, redesign of an existing protein for greater stability, increased binding affinity between two proteins, and the design of novel enzymes.
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crystal structure of Top7
Comparison of the computationally designed model (blue) to the solved x-ray structure (red) of Top7 reveals a backbone RMSD of only 1.17A.